BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from Human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
纯化方法
Immunoaffinity Chromatography using peptide coupled to agarose beads.
免疫原
Synthetic peptide corresponding aa 1-17 of Human RAD54 protein.
Suitable for use in ELISA and by Western blot. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by Westernblotting in the appropriate cell lysate or extract. Splice variants exist for this protein thatmay result in the detection of lower molecular weight bands. Recommended Dilutions: ELISA: 1/10000-1/40000. Western Blot: 1/500-1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.Synonyms: DNA repair and recombination protein RAD54-like, RAD54 homolog, RAD54A, hHR54, hRAD54