This antibody is directed against human UBP43 protein.
交叉反应 (详细)
Species reactivity (expected):A BLAST analysis was used to suggest reactivity with this protein from chimpanzee and orangutan based on 100 % homology for the immunogen sequence. Species reactivity (tested):Human.
纯化方法
Immunoaffinity chromatography
免疫原
Synthetic peptide corresponding aa 40-49 of human UBP43 protein
ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 500 - 1: 2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.9 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.Synonyms: 43 kDa ISG15-specific protease, ISG15-specific-processing protease, ISG43, Ubl carboxyl-terminal hydrolase 18, Ubl thioesterase 18