This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
交叉反应 (详细)
Species reactivity (expected):Mouse, Rat and Bovine. Species reactivity (tested):Human.
纯化方法
Immunoaffinity Chromatography
免疫原
Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein
ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.56 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
SFRP1
(Secreted Frizzled-Related Protein 1 (SFRP1))
SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1