HA-Tag 抗体 (Biotin)
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- 抗原 See all HA-Tag products
- HA-Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HA-Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 序列
- YPYDVPDYA
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
- 纯化方法
- Affinity chromatography
- 免疫原
- 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide
- 亚型
- IgG
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anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated
anti-HA-Tag antibodyWB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP
anti-HA-Tag antibody适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated
anti-HA-Tag antibodyELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated
anti-HA-Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 12CA5 unconjugated
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- 应用备注
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ELISA: 1: 15,000 - 1: 150,000. Immunohistochemistry on paraffin sections: 1: 250. Immunoprecipitation: 1: 250. Western blot: 1: 10,000 (In western blotting of bacterial extracts the antibody does notcross-react with endogenous proteins).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
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- 抗原
- HA-Tag
- 别名
- HA Epitope Tag (YPYDVPDYA) (HA-Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
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