Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification, dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 μm filtered, aseptically filled
ELISA: 1: 10,000 to 1: 50,000 (1.0 μg of Mouse Serum Albumin in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] MX and TMB asa substrate for 30 minutes at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.