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GFP 抗体 (Biotin)

Name: GFP 抗体 (Biotin)
SKU: ABIN401200
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IHC (fro), EIA 宿主: 兔 Polyclonal Biotin

产品编号 ABIN401200

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
175

15

11

9
Aequorea victoria
宿主
64

63

34

13

9

5

2

1
兔
Compatible Secondaries
克隆类型
113

73
多克隆
标记
82

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
应用范围
148

80

47

34

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

交叉反应（详细）

Ads. to Hu, Ms, Rt Serum Proteins

产品特性

Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

纯化方法

Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria

亚型

IgG

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
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GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

应用备注

ELISA: 1: 12,000 - 1: 60,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry: 1: 500 - 1: 3,000.

限制

仅限研究用
溶解方式

Resuspend product with 0.1 mL of deionized water (or equivalent).

浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

储存条件

4 °C/-20 °C

储存方法

Store vial at 2-8 °C prior to restoration. Following restoration product can be stored undiluted at 2-8 °C for one month or (in aliquots) at-20 °C or below.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein

基因ID

6100

UniProt

P42212