Reactivity occurs against Human HR23B protein. Cross reactivity with HR23B protein from Mouse and Rat may also occur as sequence homology varies by one amino acid residue in this sequence by BLAST analysis. Reactivity with HR23B protein from other sources is not known.
交叉反应 (详细)
Species reactivity (expected):Canine, Chimpanzee, S.cerevisiae. Species reactivity (tested):Human.
纯化方法
Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase
免疫原
Synthetic peptide corresponding aa 163-176 of human HR23B protein
ELISA: 1/2000 - 1/10000. Western Blot: 1/500 - 1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.1 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein