Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)
特异性
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Suitable for use in ELISA and Western blotting. Reactivity is also expected inNeutralizations, Radioimmunoassay and Immunohistochemistry. By western blot a band approximately 15.0 kDa in size corresponding to Human IL17-Fprotein is expected in the appropriate cell lysate or extract. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.1 mL of deionized water (or equivalent).
浓度
1.0 mg/mL.
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1.Synonyms: Cytokine ML-1, IL-17F, IL-24, IL24, Interleukin-24