HRP 抗体
HRP
适用: Horseradish
WB, IP, EIA, DB
宿主: 绵羊
Polyclonal
unconjugated
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- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This HRP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)
- 免疫原
- Peroxidase [Horseradish]
- 亚型
- IgG
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish WB, ELISA, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
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Western Blot: 1/1000 - 1/4000. Dot Blot. ELISA: 1/10000 - 1/40000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 85.0 mg/mL (by Refractometry)
- 缓冲液
- 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, None, None
- 储存液
- Azide free
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- Horseradish Peroxidase / HRP (HRP 产品)
- 背景
- Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
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