This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Cross-reactivity with AKT2 and AKT3 has not been determined.
交叉反应 (详细)
Species reactivity (expected):Mouse, Chimpanzee, Rat. Species reactivity (tested):Human.
产品特性
Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
纯化方法
Protein A Chromatography
免疫原
Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein
ELISA: 1/20,000. Immunoprecipitation. Immunoflourescence: 1/500-1/3,000. Western blot: 1/500-1/3,000 (Expect a band approximately 56 kDa in size corresponding tophosphorylated AKT protein by in the appropriate cell lysate or extract). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 20 μg/mL (Nopre-treatment of sample is required). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.02 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase