CD34 抗体
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- 抗原 See all CD34 抗体
- CD34
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适用
- 人, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD34 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- Recognizes the Mouse CD34 cell surface glycoprotein. Mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.
- 交叉反应 (详细)
- Species reactivity (tested):Mouse.
- 产品特性
- Synonyms: Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34
- 纯化方法
- Protein G Chromatography.
- 克隆位点
- MEC 14-7
- 亚型
- IgG2a
- Top Product
- Discover our top product CD34 Primary Antibody
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- 应用备注
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Immunoprecipitation: 2-10 μg/mL. Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one monthor (Add 40-50% Sterile Glycerol) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD34
- 别名
- CD34 (CD34 产品)
- 背景
- The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
- 基因ID
- 12490
- UniProt
- Q64314
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