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CRE Recombinase (CRE) 抗体

CRE 适用: Bacteriophage P1 WB, ELISA 宿主: 小鼠 Monoclonal 1D8H3 unconjugated
产品编号 ABIN398566
发货至: 中国
  • 抗原 See all CRE Recombinase (CRE) 抗体
    CRE Recombinase (CRE)
    适用
    • 3
    • 1
    • 1
    Bacteriophage P1
    宿主
    • 3
    • 3
    • 2
    小鼠
    克隆类型
    • 5
    • 3
    单克隆
    标记
    • 8
    非结合性
    应用范围
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    特异性
    Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.
    交叉反应 (详细)
    Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.
    纯化方法
    Protein A affinity column
    免疫原
    Purified recombinant Cre recombinase protein
    克隆位点
    1D8H3
    亚型
    IgG2a
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mL Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Lin, Liu, Sun, Yuan, Zhang, Chen: "Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line." in: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 2, pp. 114-21, (2013) (PubMed).

    Kaushik, Arias, Kwon, Lopez, Athonvarangkul, Sahu, Schwartz, Pessin, Singh: "Loss of autophagy in hypothalamic POMC neurons impairs lipolysis." in: EMBO reports, Vol. 13, Issue 3, pp. 258-65, (2012) (PubMed).

  • 抗原
    CRE Recombinase (CRE)
    别名
    Cre Recombinase (CRE 产品)
    别名
    cyclization recombinase antibody, cre antibody
    物质类
    Phage Protein
    背景
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of Cre recombinase in different protein expression systems.
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