HBxAg 抗体
HBxAg
适用: Hepatitis B Virus (HBV)
WB, ELISA, IHC (p), IHC (fro), IHC
宿主: 小鼠
Monoclonal
3F6-G10
unconjugated
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- 抗原 See all HBxAg products
- HBxAg (Hepatitis B Virus X Antigen (HBxAg))
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适用
- Hepatitis B Virus (HBV)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HBxAg antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
- 特异性
- Recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.
- 纯化方法
- Affinity purified
- 免疫原
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HB-Xag-Protein A Fusion protein.
Type of Immunogen: Fusion protein - 克隆位点
- 3F6-G10
- 亚型
- IgG2a
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anti-Hepatitis B Virus X Antigen (HBxAg) antibody
HBxAg 适用: Hepatitis B Virus (HBV) WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:100 - 1:500)
Usage: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. - 说明
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Target Species of Antibody: Hepatitis B Virus
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.09 % sodium azide, 0.1 % BSA.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Store undiluted.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.
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- 抗原
- HBxAg (Hepatitis B Virus X Antigen (HBxAg))
- 别名
- Hepatitis B X Antigen (HBxAg) (HBxAg 产品)
- 物质类
- Viral Protein
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