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PIK3C3 抗体 (N-Term)

Name: PIK3C3 抗体 (N-Term)
SKU: ABIN392555
Availability: OutOfStock

PIK3C3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB1695 unconjugated

Western blot analysis of lysates from Hela, SH-SY5Y cell line (from left to right), using PI3KC3 Antibody (N-term) A. (PIK3C3 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PIK3C3 抗体 (N-Term))

IHC (p)

Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term) (ABIN392555 and ABIN2842102) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PIK3C3 抗体 (N-Term))

3 images

(9 references)

产品编号 ABIN392555

发货至: 中国

Datasheet as PDF

抗原 See all PIK3C3 抗体

PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))
抗原表位
22

22

18

16

13

12

12

8

7

7

7

7

6

6

6

6

6

6

6

6

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 24-53, N-Term
适用
185

59

53

2

1
人
宿主
183

2
兔
Compatible Secondaries
克隆类型
176

9
多克隆
标记
51

20

19

19

15

15

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

1
This PIK3C3 antibody is un-conjugated
应用范围
146

107

82

40

39

22

22

17

16

15

10

9

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

M, Pig, Rat, X

纯化方法

This antibody is purified through a protein G column, followed by dialysis against PBS.

免疫原

This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.

克隆位点

RB1695

亚型

Ig Fraction

Top Product

Discover our top product PIK3C3 Primary Antibody
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 631-885) antibody
PIK3C3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (pSer282) antibody
PIK3C3 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (pSer164) antibody
PIK3C3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 14-39) antibody
PIK3C3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11895 unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 301-400) antibody
PIK3C3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 700-850), (Catalytic Subunit) antibody
PIK3C3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (N-Term) antibody (Alkaline Phosphatase (AP))
PIK3C3 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (N-Term) antibody (APC)
PIK3C3 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal APC

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 150-250) antibody
PIK3C3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) antibody
PIK3C3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Wang, Li, Du, Xu, Wang, Zhang, Xu, Zeng, Mao, Cao: "The Class I PI3K inhibitor S14161 induces autophagy in malignant blood cells by modulating the Beclin 1/Vps34 complex." in: Journal of pharmacological sciences, Vol. 134, Issue 4, pp. 197-202, (2018) (PubMed).

Won, Yen, Lin, Jiang-Shieh, Lin, Chen, Su: "Autophagy mediates cytotoxicity of human colorectal cancer cells treated with garcinielliptone FC." in: Journal of cellular physiology, Vol. 233, Issue 1, pp. 497-505, (2017) (PubMed).

Lin, Wang, Hsu, Cho, Yang, Chien: "Capsaicin Induces Autophagy and Apoptosis in Human Nasopharyngeal Carcinoma Cells by Downregulating the PI3K/AKT/mTOR Pathway." in: International journal of molecular sciences, Vol. 18, Issue 7, (2017) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

McLeod, Zhou, Li, Wang, He: "The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7R? surface expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 5051-61, (2011) (PubMed).

Zhou, Wang, Hasegawa, Amin, Han, Kaneko, He, Wang: "Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9424-9, (2010) (PubMed).

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, (2009) (PubMed).

Miselli, Negri, Gronchi, Losa, Conca, Brich, Fumagalli, Fiore, Casali, Pierotti, Tamborini, Pilotti: "Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?" in: Translational oncology, Vol. 1, Issue 4, pp. 177-86, (2008) (PubMed).

Liang, Feng, Ku, Dotan, Canaani, Oh, Jung: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, (2006) (PubMed).
抗原

PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

别名

PI3KC3 (PIK3C3 产品)

别名

im:7147158 antibody, wu:fc13g06 antibody, wu:fz51e12 antibody, zgc:112016 antibody, zgc:113009 antibody, VPS34 antibody, hVps34 antibody, 5330434F23Rik antibody, Vps34 antibody, phosphatidylinositol 3-kinase catalytic subunit type 3 antibody, phosphatidylinositol 3-kinase catalytic subunit type 3 L homeolog antibody, phosphatidylinositol 3-kinase, catalytic subunit type 3 antibody, PIK3C3 antibody, pik3c3.L antibody, pik3c3 antibody, Pik3c3 antibody

背景

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

分子量

101549

基因ID

5289

NCBI登录号

NP_002638

UniProt

Q8NEB9

途径

AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy