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IDH1 抗体 (AA 116-143)

Name: IDH1 抗体 (AA 116-143)
SKU: ABIN391660
Availability: OutOfStock

IDH1 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB18238 unconjugated

All lanes : Anti-IDH1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. (IDH1 抗体 (AA 116-143))

Immunohistochemical analysis of paraffin-embedded H. (IDH1 抗体 (AA 116-143))

IHC (p)

Confocal immunofluorescent analysis of IDH1 Antibody (Center) (ABIN391660 and ABIN2841571) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (IDH1 抗体 (AA 116-143))

Western blot analysis of IDH1 Antibody (Center) (ABIN391660 and ABIN2841571) in HepG2 cell line and mouse liver tissue lysates (35 μg/lane). (IDH1 抗体 (AA 116-143))

Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center) (ABIN391660 and ABIN2841571). (IDH1 抗体 (AA 116-143))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (IDH1 抗体 (AA 116-143))

IHC (p)

IDH1 Antibody (Center) (ABIN391660 and ABIN2841571) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). (IDH1 抗体 (AA 116-143))

FACS

7 images

产品编号 ABIN391660

发货至: 中国

Datasheet as PDF

抗原 See all IDH1 抗体

IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
抗原表位
15

12

9

5

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 116-143
适用
97

57

41

7

7

5

5

5

5

4

4

4

3

2

2

1

1
人, 小鼠
宿主
77

36

3

1
兔
Compatible Secondaries
克隆类型
78

39
多克隆
标记
62

10

9

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This IDH1 antibody is un-conjugated
应用范围
83

34

31

27

20

16

13

13

9

6

5

2

2

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

B, Rat, Sh

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.

克隆位点

RB18238

亚型

Ig Fraction

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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (Center) antibody
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibody
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 63-90) antibody
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 74-333) antibody
IDH1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
抗原

IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

别名

IDH1 (IDH1 产品)

别名

IDCD antibody, IDH antibody, IDP antibody, IDPC antibody, PICD antibody, NADP-CICDH antibody, AI314845 antibody, AI788952 antibody, E030024J03Rik antibody, Id-1 antibody, Idh-1 antibody, Idpc antibody, cb876 antibody, fm90e09 antibody, im:7143416 antibody, wu:fm90e09 antibody, F23E12.180 antibody, F23E12_180 antibody, IDH-I antibody, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 antibody, isocitrate dehydrogenase 1 antibody, isocitrate dehydrogenase I antibody, isocitrate dehydrogenase (NADP(+)) 1, cytosolic antibody, isocitrate dehydrogenase 1 (NADP+), soluble antibody, isocitrate dehydrogenase 1 (NADP+) L homeolog antibody, isocitrate dehydrogenase 1 antibody, IDH1 antibody, Idh1 antibody, idh1.L antibody, idh1 antibody

背景

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

分子量

46659

基因ID

3417

NCBI登录号

NP_001269315, NP_001269316, NP_005887

UniProt

O75874

途径

Warburg Effect