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SPHK2 抗体 (C-Term)

Name: SPHK2 抗体 (C-Term)
SKU: ABIN391349
Availability: OutOfStock

This anti-SPHK2 antibody is a 兔多克隆 antibody detecting SPHK2 in WB 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 2+ publications.

All lanes : Anti-hSPHK2- at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 μg per lane. (SPHK2 抗体 (C-Term))

Immunohistochemical analysis of B on paraffin-embedded Human kidney tissue was performed on the Leica®BOND RXm. (SPHK2 抗体 (C-Term))

IHC (p)

SPHK2 Antibody (ABIN391349 and ABIN2841370) western blot analysis in HepG2 cell line lysates (35 μg/lane). (SPHK2 抗体 (C-Term))

Immunohistochemical analysis of B on paraffin-embedded Human brain tissue was performed on the Leica®BOND RXm. (SPHK2 抗体 (C-Term))

IHC (p)

4 images

(2 references)

产品编号 ABIN391349

发货至: 中国

Quick Overview for SPHK2 抗体 (C-Term) (ABIN391349)

抗原

See all SPHK2 抗体

SPHK2 (Sphingosine Kinase 2 (SPHK2))

适用

人

宿主

兔

克隆类型

多克隆

标记

This SPHK2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB03625

抗原表位
10

8

6

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 590-620, C-Term

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.

亚型

IgG
anti-Sphingosine Kinase 2 (SPHK2) (AA 1-654) antibody
SPHK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (AA 101-200) antibody
SPHK2 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (N-Term) antibody
SPHK2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) antibody
SPHK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (AA 1-654) antibody
SPHK2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (Tyr754) antibody
SPHK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (AA 1-30), (N-Term) antibody
SPHK2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03623 unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (AA 1-30) antibody
SPHK2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) (AA 590-620) antibody
SPHK2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 2 (SPHK2) antibody
SPHK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. IHC-P-Leica: 1:250. IHC-P-Leica: 1:250

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Sun, Zhang, Wang, Wang, Ma, Lei, Zhou, Sun, Lu, Liu, Han: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology, (2015) (PubMed).

Coste, Brenneis, Linke, Pierre, Maeurer, Becker, Schmidt, Gao, Geisslinger, Scholich: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, (2008) (PubMed).
抗原

SPHK2 (Sphingosine Kinase 2 (SPHK2))

别名

SPHK2

背景

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

分子量

69217

NCBI登录号

NP_001191087, NP_001191088, NP_001191089, NP_064511

UniProt

Q9NRA0

途径

VEGF Signaling