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SPHK1 抗体 (AA 286-315)

Name: SPHK1 抗体 (AA 286-315)
SKU: ABIN391346
Availability: OutOfStock

SPHK1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03621 unconjugated

Anti-SPHK1 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 μg per lane. (SPHK1 抗体 (AA 286-315))

IHC (p)

Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (ABIN391346 and ABIN2841367). (SPHK1 抗体 (AA 286-315))

All lanes : Anti-SPHK1 Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 μg per lane. (SPHK1 抗体 (AA 286-315))

IHC (p)

5 images

(22 references)

产品编号 ABIN391346

发货至: 中国

Datasheet as PDF

抗原 See all SPHK1 抗体

SPHK1 (Sphingosine Kinase 1 (SPHK1))
抗原表位
15

9

8

7

7

7

6

6

6

6

6

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 286-315
适用
100

46

42

9

3

2

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
92

8

1
兔
Compatible Secondaries
克隆类型
88

13
多克隆
标记
50

7

5

4

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1
This SPHK1 antibody is un-conjugated
应用范围
69

41

24

18

13

13

11

10

4

3

3

2

2

2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.

克隆位点

RB03621

亚型

Ig Fraction

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SPHK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D6 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 313-343) antibody
SPHK1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
SPHK1 适用: 人, 小鼠, 大鼠, 灵长类 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 148-398) antibody
SPHK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-384) antibody
SPHK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 50-384) antibody
SPHK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-30), (N-Term) antibody
SPHK1 适用: 人 WB 宿主: 兔 Polyclonal RB3619 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-30) antibody
SPHK1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 59-89) antibody
SPHK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (C-Term) antibody
SPHK1 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Furuya, Shimizu, Tamashiro, Iino, Bielawski, Chan, Pagano, Kawamori: "Sphingosine kinase 1 expression enhances colon tumor growth." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 120, (2018) (PubMed).

Song, Zhang, Chen, Xia, Qin, Waeber, Sheng: "Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain." in: Cell death & disease, Vol. 8, Issue 7, pp. e2912, (2018) (PubMed).

Zhu, You, Tan, Li, Qiu, Li, Huang, Zheng, Ren: "Overexpression of sphingosine kinase 1 is predictive of poor prognosis in human breast cancer." in: Oncology letters, Vol. 14, Issue 1, pp. 63-72, (2017) (PubMed).

Do, Kim, Kim, Lee, Do, Kim, Chae, Sohn: "Predictive Value of Sphingosine Kinase 1 Expression in Papillary Thyroid Carcinoma." in: Anticancer research, Vol. 37, Issue 10, pp. 5399-5405, (2017) (PubMed).

Gairhe, Joshi, Bastola, McLendon, Oka, Fagan, McMurtry: "Sphingosine-1-phosphate is involved in the occlusive arteriopathy of pulmonary arterial hypertension." in: Pulmonary circulation, Vol. 6, Issue 3, pp. 369-80, (2016) (PubMed).

Dick, Hengst, Fox, Colledge, Kale, Sung, Sharma, Amin, Loughran, Kester, Wang, Yun: "The apoptotic mechanism of action of the sphingosine kinase 1 selective inhibitor SKI-178 in human acute myeloid leukemia cell lines." in: The Journal of pharmacology and experimental therapeutics, Vol. 352, Issue 3, pp. 494-508, (2015) (PubMed).

Zheng, Wei, Wang, Xu, Xiao, Liu, Jia, Cheng: "Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia." in: Experimental neurology, (2015) (PubMed).

Matula, Collie-Duguid, Murray, Parikh, Grabsch, Tan, Lalwani, Garau, Ong, Bain, Smith, Urquhart, Bielawski, Finnegan, Petty: "Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer." in: BMC cancer, Vol. 15, pp. 762, (2015) (PubMed).

Xiong, Wang, Guan, Wu, Xu, Wang, Rong, Huang, Huang, Liao, Fu, Yuan: "SphK1 confers resistance to apoptosis in gastric cancer cells by downregulating Bim via stimulating Akt/FoxO3a signaling." in: Oncology reports, Vol. 32, Issue 4, pp. 1369-73, (2014) (PubMed).

Altay, Suzuki, Hasegawa, Caner, Krafft, Fujii, Tang, Zhang: "Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2513-6, (2012) (PubMed).

Fischer, Alliod, Martinier, Newcombe, Brana, Pouly: "Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions." in: PLoS ONE, Vol. 6, Issue 8, pp. e23905, (2011) (PubMed).

Song, Xiong, Li, Liao, Wang, Wu, Li: "Sphingosine kinase-1 enhances resistance to apoptosis through activation of PI3K/Akt/NF-?B pathway in human non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 7, pp. 1839-49, (2011) (PubMed).

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury." in: Anesthesiology, Vol. 114, Issue 2, pp. 363-73, (2011) (PubMed).

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction." in: American journal of physiology. Renal physiology, Vol. 300, Issue 1, pp. F167-76, (2011) (PubMed).

Nayak, Huo, Kwang, Pushparaj, Kumar, Ling, Dheen: "Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia." in: Neuroscience, Vol. 166, Issue 1, pp. 132-44, (2010) (PubMed).

Liu, Zheng, Zhang, Wu, Xiong, Li, Song: "Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy." in: BMC cancer, Vol. 10, pp. 495, (2010) (PubMed).

Crousillac, Colonna, McMains, Dewey, Gleason: "Sphingosine-1-phosphate elicits receptor-dependent calcium signaling in retinal amacrine cells." in: Journal of neurophysiology, Vol. 102, Issue 6, pp. 3295-309, (2009) (PubMed).

Wadgaonkar, Patel, Grinkina, Romano, Liu, Zhao, Sammani, Garcia, Natarajan: "Differential regulation of sphingosine kinases 1 and 2 in lung injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L603-13, (2009) (PubMed).

Li, Yu, Xia, Zhang, Weng, Zheng, Kong, Hu, Zeng, Zeng, Li, Li, Song: "Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 4, pp. 1393-9, (2009) (PubMed).

Li, Guan, Gong, Song, Zhang, Wu, Yuan, Zheng, Huang, Li: "Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 21, pp. 6996-7003, (2008) (PubMed).

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抗原

SPHK1 (Sphingosine Kinase 1 (SPHK1))

别名

SPHK1 (SPHK1 产品)

别名

CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody

背景

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

基因ID

8877

NCBI登录号

NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt

Q9NYA1

途径

VEGF Signaling