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SPHK1 抗体 (N-Term)

Name: SPHK1 抗体 (N-Term)
SKU: ABIN391345
Availability: OutOfStock

SPHK1 适用: 人 WB 宿主: 兔 Polyclonal RB3619 unconjugated

Western blot analysis of SPHK1-M1 Antibody (arrow) using rabbit polyclonal SPHK1-M1 Antibody (ABIN391345 and ABIN2841366). (SPHK1 抗体 (N-Term))

Western blot analysis of lysate from A431 cell line, using SPHK1 Antibody (M1) (ABIN391345 and ABIN2841366). (SPHK1 抗体 (N-Term))

The anti-SphK1 Pab (ABIN391345 and ABIN2841366) is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). (SPHK1 抗体 (N-Term))

Western blot analysis of anti-hSPHK1-M1 Pab (ABIN391345 and ABIN2841366) in 293 cell line lysates transiently transfected with the SPHK1 gene (2 μg/lane). (SPHK1 抗体 (N-Term))

4 images

(2 references)

产品编号 ABIN391345

发货至: 中国

Datasheet as PDF

抗原 See all SPHK1 抗体

SPHK1 (Sphingosine Kinase 1 (SPHK1))
抗原表位
15

9

7

7

7

7

7

6

6

6

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1-30, N-Term
适用
100

47

43

9

3

2

2

2

1

1

1

1
人
宿主
92

8

1
兔
Compatible Secondaries
克隆类型
88

13
多克隆
标记
50

7

5

4

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1
This SPHK1 antibody is un-conjugated
应用范围
69

41

24

18

13

13

12

10

4

3

3

2

2

2
Western Blotting (WB)

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK1.

克隆位点

RB3619

亚型

Ig Fraction

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anti-Sphingosine Kinase 1 (SPHK1) (AA 1-384) antibody
SPHK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D6 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
SPHK1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03621 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 313-343) antibody
SPHK1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
SPHK1 适用: 人, 小鼠, 大鼠, 灵长类 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 148-398) antibody
SPHK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-384) antibody
SPHK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 50-384) antibody
SPHK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-30) antibody
SPHK1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 59-89) antibody
SPHK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (C-Term) antibody
SPHK1 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:500

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Hayashi, Nakagami, Takami, Koriyama, Mori, Tamai, Sun, Nagao, Morishita, Kaneda: "FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 6, pp. 909-14, (2009) (PubMed).

Gellings Lowe, Swaney, Moreno, Sabbadini: "Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-beta-stimulated collagen production by cardiac fibroblasts." in: Cardiovascular research, Vol. 82, Issue 2, pp. 303-12, (2009) (PubMed).
抗原

SPHK1 (Sphingosine Kinase 1 (SPHK1))

别名

SPHK1 (SPHK1 产品)

别名

CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody

背景

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

基因ID

8877

NCBI登录号

NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt

Q9NYA1

途径

VEGF Signaling