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CKM 抗体 (C-Term)

Name: CKM 抗体 (C-Term)
SKU: ABIN391096
Availability: OutOfStock

CKM 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB5382 unconjugated

The anti-CKM Pab (ABIN391096 and ABIN2841235) is used in Western blot to detect CKM in C6 cell lysate. (CKM 抗体 (C-Term))

Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). (CKM 抗体 (C-Term))

FACS

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (CKM 抗体 (C-Term))

IHC (p)

3 images

(1 reference)

产品编号 ABIN391096

发货至: 中国

Datasheet as PDF

抗原 See all CKM 抗体

CKM (Creatine Kinase, Muscle (CKM))
抗原表位
15

12

11

8

7

6

3

3

2

1

1

1

1

1

1
AA 283-313, C-Term
适用
99

55

38

17

9

6

6

4

3

3

3

2

1

1
人
宿主
100

20

14

1

1
兔
Compatible Secondaries
克隆类型
115

20
多克隆
标记
65

21

13

8

6

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CKM antibody is un-conjugated
应用范围
98

65

48

18

18

13

13

13

11

9

8

7

7

5

2

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

预测反应

B, Pig, Rb

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM.

克隆位点

RB5382

亚型

Ig Fraction

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CKM 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 11-367) antibody
CKM 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 11-367) antibody
CKM 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 282-381) antibody
CKM 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E3 unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 1-381) antibody
CKM 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 2F3-B11 unconjugated

anti-Creatine Kinase, Muscle (CKM) (N-Term) antibody
CKM 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 1-381) antibody
CKM 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1D10F2 unconjugated

anti-Creatine Kinase, Muscle (CKM) (N-Term) antibody
CKM 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (AA 11-367) antibody
CKM 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Creatine Kinase, Muscle (CKM) (N-Term) antibody
CKM 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Yang, Fann, Chang, Tai, Jiang, Kao: "Regulation of sodium-calcium exchanger activity by creatine kinase under energy-compromised conditions." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 28275-85, (2010) (PubMed).
抗原

CKM (Creatine Kinase, Muscle (CKM))

别名

CKM (CKM 产品)

别名

CKMM antibody, M-CK antibody, Ckmm antibody, MCK antibody, ckmm antibody, m-ck antibody, CKM antibody, CK-M antibody, cb51 antibody, ckm antibody, mck antibody, wu:fa28d05 antibody, ckm3 antibody, wu:fb55e09 antibody, zgc:64204 antibody, zgc:92070 antibody, creatine kinase, M-type antibody, creatine kinase, muscle antibody, creatine kinase, M-type L homeolog antibody, creatine kinase, muscle a antibody, creatine kinase, muscle b antibody, CKM antibody, Ckm antibody, ckm.L antibody, ckm antibody, ckma antibody, ckmb antibody

背景

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

分子量

43101

基因ID

1158

NCBI登录号

NP_001815

UniProt

P06732