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HSPD1 抗体 (N-Term)

Name: HSPD1 抗体 (N-Term)
SKU: ABIN390772
Availability: OutOfStock

HSPD1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB19532 unconjugated

All lanes : Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. (HSPD1 抗体 (N-Term))

IHC (p)

All lanes : Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: K562 whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: Hela whole cell lysates Lysates/proteins at 20 μg per lane. (HSPD1 抗体 (N-Term))

Western blot analysis of Hsp 60 Antibody (N-term) (ABIN390772 and ABIN2841030) in mouse lung tissue lysates (35 μg/lane). (HSPD1 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (HSPD1 抗体 (N-Term))

IHC (p)

5 images

产品编号 ABIN390772

发货至: 中国

Datasheet as PDF

抗原 See all HSPD1 抗体

HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
抗原表位
13

13

10

4

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 80-109, N-Term
适用
207

122

118

74

71

69

64

63

61

61

46

41

36

26

25

21

17

17

14

14

13

10

10

10

10

10

4

4

4

4

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
人, 小鼠
宿主
119

114

3
兔
Compatible Secondaries
克隆类型
123

111
多克隆
标记
112

19

10

7

7

7

7

6

6

6

5

5

5

5

5

5

4

3

2

2

2

2

2

2
This HSPD1 antibody is un-conjugated
应用范围
185

103

98

90

65

63

27

27

24

23

12

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This Hsp 60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human Hsp 60.

克隆位点

RB19532

亚型

Ig Fraction

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anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
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HSPD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibody
HSPD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 316-573) antibody
HSPD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibody
HSPD1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Hamster, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, FACS, IF, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated

应用备注

WB: 1:2000. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
抗原

HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

别名

Hsp 60 (HSPD1 产品)

别名

CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, chaperonin antibody, cpn60 antibody, groel antibody, hld4 antibody, hsp60 antibody, hsp65 antibody, spg13 antibody, 60kDa antibody, Hsp60 antibody, 12 antibody, BP5 antibody, CG12101 antibody, Cpn60 antibody, Dmel\\CG12101 antibody, Dmhsp60 antibody, G62 antibody, HSP60A antibody, Hsp60A antibody, IEF16 antibody, Mmp-P1 antibody, SSP 7506 antibody, d-hsp60 antibody, hsp60A antibody, l(1)10Ac antibody, l(1)BP5 antibody, l(1)G8 antibody, l(1)HM21 antibody, l(1)L12 antibody, l(1)dp025 antibody, cb863 antibody, fa04a05 antibody, fb22d10 antibody, fi27b05 antibody, id:ibd2197 antibody, sb:cb144 antibody, wu:fa04a05 antibody, wu:fb22d10 antibody, wu:fi04a12 antibody, wu:fi27b05 antibody, MIF4 antibody, MNA2 antibody, mopA antibody, groL antibody, crpA antibody, Hspd1-30p antibody, heat shock protein family D (Hsp60) member 1 antibody, heat shock protein family D (Hsp60) member 1 S homeolog antibody, 60 kDa heat shock protein, mitochondrial antibody, heat shock protein 1 (chaperonin) antibody, Heat shock protein 60A antibody, heat shock 60 protein 1 antibody, chaperone ATPase HSP60 antibody, molecular chaperone GroEL antibody, thermosome subunit antibody, chaperonin GroEL antibody, mitochondrial chaperonin antibody, heat shock protein family D member 1 antibody, HSPD1 antibody, hspd1.S antibody, hspd1 antibody, LOC100414401 antibody, Hspd1 antibody, Hsp60A antibody, HSP60 antibody, groEL antibody, MMP_RS07785 antibody, groEl antibody, LOC100136430 antibody

背景

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species.The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.

分子量

54794

基因ID

51182

NCBI登录号

NP_057383

UniProt

Q0VDF9

途径

Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity