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CD14 抗体 (N-Term)

Name: CD14 抗体 (N-Term)
SKU: ABIN390260
Availability: OutOfStock

CD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated

Overlay histogram showing Jurkat cells stained with A(green line). (CD14 抗体 (N-Term))

FACS

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0. (CD14 抗体 (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lane 3: A549 whole cell lysate Lysates/proteins at 20 μg per lane. (CD14 抗体 (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 μg per lane. (CD14 抗体 (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: Human lung tissue lysate Lane 3: Human liver tissue lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 μg per lane. (CD14 抗体 (N-Term))

All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human lung lysate Lysates/proteins at 20 μg per lane. (CD14 抗体 (N-Term))

Immunohistochemical analysis of paraffin-embedded human liver tissue using A performed on the Leica® BOND RXm. (CD14 抗体 (N-Term))

IHC (p)

7 images

(1 reference)

产品编号 ABIN390260

发货至: 中国

Datasheet as PDF

抗原 See all CD14 抗体

CD14
抗原表位
9

6

5

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 54-83, N-Term
适用
221

38

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
人
宿主
192

51

16

3

1
兔
Compatible Secondaries
克隆类型
210

53
多克隆
标记
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
应用范围
196

78

56

50

45

43

20

13

12

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.

克隆位点

RB14105

亚型

IgG

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应用备注

IF: 1:25. WB: 1:2000. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:1000. FC: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Ishida, Akita, Mori, Tanida, Toda, Inoue, Nakada: "Negative regulation of Toll-like receptor-4 signaling through the binding of glycosylphosphatidylinositol-anchored glycoprotein, CD14, with the sialic acid-binding lectin, CD33." in: The Journal of biological chemistry, Vol. 289, Issue 36, pp. 25341-50, (2014) (PubMed).
抗原

CD14

别名

CD14 (CD14 产品)

别名

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

背景

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

分子量

40076

基因ID

929

NCBI登录号

NP_000582, NP_001035110, NP_001167575, NP_001167576

UniProt

P08571

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades