CHEK1 抗体 (pSer317)
(1 reference)-
- 抗原 See all CHEK1 抗体
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
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抗原表位
- pSer317
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHEK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
- 纯化方法
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- 免疫原
- This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
- 克隆位点
- RB7845-RB18425
- 亚型
- Ig Fraction
- Top Product
- Discover our top product CHEK1 Primary Antibody
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anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibodyCHEK1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibodyCHEK1 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:1000. IHC-P: 1:50~100. DB: 1:500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- 有效期
- 6 months
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: "TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint." in: BMC molecular biology, Vol. 17, pp. 3, (2016) (PubMed).
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: "TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint." in: BMC molecular biology, Vol. 17, pp. 3, (2016) (PubMed).
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- 抗原
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- 别名
- CHK1 (CHEK1 产品)
- 背景
- Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
- 分子量
- 54434
- 基因ID
- 1111
- NCBI登录号
- NP_001107593, NP_001107594, NP_001231775, NP_001265
- UniProt
- O14757
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
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