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PCNA 抗体 (C-Term)

Name: PCNA 抗体 (C-Term)
SKU: ABIN389344
Availability: OutOfStock

PCNA 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17486 unconjugated

Western blot analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) in Jurkat, Hela, 293 cell line lysates (35 μg/lane). (PCNA 抗体 (C-Term))

PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (PCNA 抗体 (C-Term))

IHC (p)

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCNA 抗体 (C-Term))

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCNA 抗体 (C-Term))

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) (ABIN389344 and ABIN2839453). (PCNA 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) (ABIN389344 and ABIN2839453) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PCNA 抗体 (C-Term))

IHC (p)

6 images

(5 references)

产品编号 ABIN389344

发货至: 中国

Datasheet as PDF

抗原 See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
抗原表位
16

16

16

15

11

11

8

6

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 231-261, C-Term
适用
207

150

149

54

28

27

21

19

19

18

17

14

11

10

6

6

5

5

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1
人
宿主
129

91

3
兔
Compatible Secondaries
克隆类型
128

94

1
多克隆
标记
109

16

13

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
应用范围
188

68

63

60

52

46

46

40

29

15

15

11

5

4

3

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

B, Ha, Pr, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.

克隆位点

RB17486

亚型

Ig Fraction

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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 兔, 犬, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

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PCNA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H10 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Deng, Xu, Zou, Shen, Peng, Liu, Engelhardt, Yan, Qiu: "DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells." in: Journal of virology, Vol. 91, Issue 1, (2017) (PubMed).

Lei, Li, Luo: "Liuweiwuling tablets attenuate acetaminophen-induced acute liver injury and promote liver regeneration in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 26, pp. 8089-95, (2015) (PubMed).

Ding, Ko, Pehar, Kotch, Peters, Luo, Salamat, Puglielli: "Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces ?-secretase (BACE1) levels and A? generation." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8424-33, (2012) (PubMed).

Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, (2012) (PubMed).

Li, Li, Zhang, Bai, Wang, Zhao, Jonas: "Lentiviral vector-mediated PAX6 overexpression promotes growth and inhibits apoptosis of human retinoblastoma cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 11, pp. 8393-400, (2011) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA (PCNA 产品)

背景

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

分子量

28769

基因ID

5111

NCBI登录号

NP_002583, NP_872590

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy