Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
预测反应
B, C, M, Pig, Rat
纯化方法
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
免疫原
This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SFRS2.
SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.