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SQSTM1 抗体 (AA 317-346)

Name: SQSTM1 抗体 (AA 317-346)
SKU: ABIN388979
Availability: OutOfStock

SQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated

All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane. (SQSTM1 抗体 (AA 317-346))

IHC (p)

Fluorescent image of cells stained with SQSTM1 (p62) (C-term) antibody. (SQSTM1 抗体 (AA 317-346))

Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (ABIN388979 and ABIN2850435) on Methanol-fixed and PFA fixed HeLa cells. (SQSTM1 抗体 (AA 317-346))

All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. (SQSTM1 抗体 (AA 317-346))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SQSTM1 抗体 (AA 317-346))

IHC (p)

6 images

(22 references)

产品编号 ABIN388979

发货至: 中国

Datasheet as PDF

抗原 See all SQSTM1 抗体

SQSTM1 (Sequestosome 1 (SQSTM1))
抗原表位
19

17

15

13

8

7

7

7

6

6

6

5

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 317-346
适用
149

64

61

18

4

4

4

4

3

2

2

2

1

1

1

1
人, 小鼠
宿主
125

22

1

1
兔
Compatible Secondaries
克隆类型
127

22
多克隆
标记
70

11

10

7

7

6

4

4

4

4

4

4

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SQSTM1 antibody is un-conjugated
应用范围
100

56

39

37

20

16

16

15

14

13

9

5

3

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids of human SQSTM1 (p62).

克隆位点

RB4614

亚型

IgG

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anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibody
SQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibody
SQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibody
SQSTM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (C-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
SQSTM1 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A2 unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:200. IF: 1:50~100. WB: 1:2000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Tan, Wang, Yu, Zhang, Bristow, Hill, Tannock: "Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors." in: Neoplasia (New York, N.Y.), Vol. 18, Issue 6, pp. 347-55, (2017) (PubMed).

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Tan, Joshua, Wang, Bristow, Wouters, Allen, Tannock: "Up-regulation of autophagy is a mechanism of resistance to chemotherapy and can be inhibited by pantoprazole to increase drug sensitivity." in: Cancer chemotherapy and pharmacology, Vol. 79, Issue 5, pp. 959-969, (2017) (PubMed).

Tan, Joshua, Saggar, Yu, Wang, Kanga, Zhang, Chen, Wouters, Tannock: "Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy." in: British journal of cancer, Vol. 112, Issue 5, pp. 832-40, (2015) (PubMed).

Wu, Zhang, Lu, Xia, Zhou, Petrof, Claud, Chen, Chang, Carmeliet, Sun: "Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis." in: Gut, Vol. 64, Issue 7, pp. 1082-94, (2015) (PubMed).

Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, (2015) (PubMed).

Zhang, Li, Wang, Chen, Dong, Nie: "Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis." in: Tumour biology, (2015) (PubMed).

Gurpinar, Grizzle, Shacka, Mader, Li, Piazza, Russo, Keeton, Piazza: "A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy." in: Molecular cancer therapeutics, Vol. 12, Issue 5, pp. 663-74, (2013) (PubMed).

Urtishak, Edwards, Wang, Hudome, Robinson, Barrett, Cao, Cory, Moore, Bantly, Yu, Chen, Atlas, Willman, Kundu, Carroll, Heerema, Devidas, Hilden, Dreyer, Hunger, Reaman, Felix: "Potent obatoclax cytotoxicity and activation of triple death mode killing across infant acute lymphoblastic leukemia." in: Blood, Vol. 121, Issue 14, pp. 2689-703, (2013) (PubMed).

Han, Pan, Xu, Xiao, An, Tie, Pan, Li: "Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage." in: Autophagy, Vol. 8, Issue 5, pp. 812-25, (2012) (PubMed).

Soontornniyomkij, Risbrough, Young, Soontornniyomkij, Jeste, Achim: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, (2012) (PubMed).

Lee, Shin, Yuk, Shi, Choi, Lee, Huang, Kim, Kim, Lee, Jo: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, (2011) (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, (2011) (PubMed).

Zhang, Lowry, Guarente, Haimovich: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, (2010) (PubMed).

Maynard, Dvorak, Khailova, Dobrenen, Arganbright, Halpern, Kurundkar, Maheshwari, Dvorak: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, (2010) (PubMed).

Lin, Whang, Donner, Du, Lorch, He, Jiang, Price, Moore, Ruan: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, (2010) (PubMed).

Chu, Wang, Wu, Lei: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, (2010) (PubMed).

Wang, Gorvel, Chu, Wu, Lei: "Helicobacter pylori impairs murine dendritic cell responses to infection." in: PLoS ONE, Vol. 5, Issue 5, pp. e10844, (2010) (PubMed).

Kobayashi, Volden, Timm, Mao, Xu, Liang: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, (2009) (PubMed).

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抗原

SQSTM1 (Sequestosome 1 (SQSTM1))

别名

SQSTM1 (p62) (SQSTM1 产品)

别名

SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

背景

SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

分子量

47687

基因ID

8878

NCBI登录号

NP_001135770, NP_001135771, NP_003891

UniProt

Q13501

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy