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ube3a 抗体 (C-Term)

Name: ube3a 抗体 (C-Term)
SKU: ABIN388925
Availability: OutOfStock

ube3a 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB4440 unconjugated

Western blot analysis of UBE3A Antibody (C-term) (ABIN388925 and ABIN2850432) in T47D cell line lysates (35 μg/lane). (ube3a 抗体 (C-Term))

UBE3A Antibody (C-term) (ABIN388925 and ABIN2850432) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (ube3a 抗体 (C-Term))

FACS

Confocal immunofluorescent analysis of UBE3A Antibody (C-term) (ABIN388925 and ABIN2850432) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (ube3a 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ube3a 抗体 (C-Term))

IHC (p)

4 images

(1 reference)

产品编号 ABIN388925

发货至: 中国

Datasheet as PDF

抗原 See all ube3a 抗体

ube3a (Ubiquitin Protein Ligase E3A (ube3a))
抗原表位
15

6

5

3

2

2

2

1

1

1

1

1

1
AA 836-865, C-Term
适用
50

11

8

5

4

4

4

4

3

2

2

2

1

1

1

1

1
人
宿主
46

4
兔
Compatible Secondaries
克隆类型
46

4
多克隆
标记
27

4

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
This ube3a antibody is un-conjugated
应用范围
26

17

16

13

13

8

7

7

3

3

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 836-865 amino acids from the C-terminal region of human UBE3A.

克隆位点

RB4440

亚型

Ig Fraction

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anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 51-150) antibody
ube3a 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) antibody
ube3a 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) antibody
ube3a 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 300-590) antibody
ube3a 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) (Middle Region) antibody
ube3a 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马, 斑马鱼, Cow WB 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 83-234) antibody
ube3a 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) antibody
ube3a 适用: 人 IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) antibody
ube3a 适用: 人 WB, IHC, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 158-407) antibody
ube3a 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Protein Ligase E3A (ube3a) (C-Term) antibody
ube3a 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马, 斑马鱼, Cow, 小鸡, 猴, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Wang, Mitchell: "Cognition and Synaptic-Plasticity Related Changes in Aged Rats Supplemented with 8- and 10-Carbon Medium Chain Triglycerides." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160159, (2017) (PubMed).
抗原

ube3a (Ubiquitin Protein Ligase E3A (ube3a))

别名

UBE3A (ube3a 产品)

别名

ANCR antibody, AS antibody, E6-AP antibody, EPVE6AP antibody, HPVE6A antibody, DDBDRAFT_0188760 antibody, DDBDRAFT_0302447 antibody, DDB_0188760 antibody, DDB_0302447 antibody, ube3a antibody, im:7140733 antibody, zgc:92173 antibody, 4732496B02 antibody, 5830462N02Rik antibody, A130086L21Rik antibody, Hpve6a antibody, mKIAA4216 antibody, As antibody, CG6190 antibody, Dmel\\CG6190 antibody, Dube3a antibody, UBE3A antibody, dUBE3A antibody, das antibody, dube3A antibody, dube3a antibody, MGC69536 antibody, UME3A antibody, ubiquitin protein ligase E3A antibody, ubiquitin-protein ligase E3A antibody, Ubiquitin protein ligase E3A antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) antibody, UBE3A antibody, ube3a antibody, LOC100194730 antibody, Ube3a antibody, ube3a.S antibody, Eint_040430 antibody

背景

UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830], also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.

分子量

100688

基因ID

7337

NCBI登录号

NP_000453, NP_570853, NP_570854

UniProt

Q05086

途径

Intracellular Steroid Hormone Receptor Signaling Pathway