Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
预测反应
X, B
纯化方法
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
免疫原
This USP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human USP12.
UBH1 antibody, USP12L1 antibody, usp12 antibody, USP12P1 antibody, sb:eu882 antibody, wu:fi09d12 antibody, Ubh1 antibody, ubh1 antibody, usp12l1 antibody, ubiquitin specific peptidase 12 antibody, ubiquitin specific peptidase 12a antibody, ubiquitin specific peptidase 12 L homeolog antibody, ubiquitin specific peptidase 12 S homeolog antibody, USP12 antibody, usp12 antibody, Usp12 antibody, usp12a antibody, usp12.L antibody, usp12.S antibody
背景
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.