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ATG16L1 抗体 (C-Term)

Name: ATG16L1 抗体 (C-Term)
SKU: ABIN388543
Availability: OutOfStock

ATG16L1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB7491 unconjugated

Western blot analysis of hG16L- 1817c in NIH-3T3, HepG2, Hela, Jurkat and NCI- cell line lysates (35 μg/lane). (ATG16L1 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy G16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ATG16L1 抗体 (C-Term))

IHC (p)

2 images

(1 reference)

产品编号 ABIN388543

发货至: 中国

Datasheet as PDF

抗原 See all ATG16L1 抗体

ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))
抗原表位
16

6

5

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 454-483, C-Term
适用
77

49

32

11

6

6

6

6

6

4

3

2

1

1
人
宿主
80

11

1
兔
Compatible Secondaries
克隆类型
75

17
多克隆
标记
48

5

5

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG16L1 antibody is un-conjugated
应用范围
66

29

24

15

14

12

10

10

6

4

3

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-483 amino acids from the C-terminal region of human ATG16L.

克隆位点

RB7491

亚型

Ig Fraction

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anti-ATG16 Autophagy Related 16-Like 1 (ATG16L1) (AA 501-607) antibody
ATG16L1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-ATG16 Autophagy Related 16-Like 1 (ATG16L1) (AA 90-300) antibody
ATG16L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG16 Autophagy Related 16-Like 1 (ATG16L1) (AA 11-257) antibody
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anti-ATG16 Autophagy Related 16-Like 1 (ATG16L1) (pSer287) antibody
ATG16L1 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Gao, Kang, Liao, Ding, Gambotto, Watkins, Liu, Stolz, Yin: "Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1371-83, (2010) (PubMed).
抗原

ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

别名

ATG16L (ATG16L1 产品)

别名

APG16L antibody, ATG16A antibody, ATG16L antibody, IBD10 antibody, WDR30 antibody, 1500009K01Rik antibody, Apg16l antibody, Atg16l antibody, Wdr30 antibody, atg16 antibody, atg16l antibody, fb57e05 antibody, wu:fb57e05 antibody, zgc:110147 antibody, autophagy related 16 like 1 antibody, autophagy related 16-like 1 (S. cerevisiae) antibody, autophagy related 16-like 1 antibody, autophagy related 16 like 1 S homeolog antibody, ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody, ATG16L1 antibody, Atg16l1 antibody, atg16l1 antibody, atg16l1.S antibody

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

分子量

68265

基因ID

55054

NCBI登录号

NP_001177195, NP_001177196, NP_060444, NP_110430, NP_942593

UniProt

Q676U5

途径

Autophagy