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ATG12 抗体 (N-Term)

Name: ATG12 抗体 (N-Term)
SKU: ABIN388536
Availability: OutOfStock

ATG12 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7475 unconjugated

All lanes : Anti-G12L Antibody (E3) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (ATG12 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy G12L antibody (N-term) 1816a , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ATG12 抗体 (N-Term))

IHC (p)

Fluorescent image of cells stained with ATG12 (N-term) antibody. (ATG12 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ATG12 抗体 (N-Term))

IHC (p)

4 images

(5 references)

产品编号 ABIN388536

发货至: 中国

Datasheet as PDF

抗原 See all ATG12 抗体

ATG12 (Autophagy Related 12 (ATG12))
抗原表位
15

12

8

7

6

6

6

3

2

2

1

1

1

1

1

1

1

1

1
AA 1-30, N-Term
适用
78

36

26

6

6

6

6

5

4

4

4

4

2

2

2

2

2

2

2

2

1
人
宿主
75

19

2
兔
Compatible Secondaries
克隆类型
73

23
多克隆
标记
48

7

6

6

6

4

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG12 antibody is un-conjugated
应用范围
73

41

29

28

21

13

13

9

8

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.

克隆位点

RB7475

亚型

Ig Fraction

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anti-Autophagy Related 12 (ATG12) (AA 1-74) antibody
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anti-Autophagy Related 12 (ATG12) (AA 79-128) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 猴, 小鸡, 兔, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 12 (ATG12) (N-Term) antibody (HRP)
ATG12 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP

应用备注

IF: 1:200. WB: 1:2000. IHC-P: 1:50~100. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Yang, Zhao, Mei, Jiang, Geng, Li, Yao, Liu, Kong, Cao: "HMGA2 plays an important role in Cr (VI)-induced autophagy." in: International journal of cancer, Vol. 141, Issue 5, pp. 986-997, (2017) (PubMed).

Chen, Cao, Zhou, Liu, Che, Mizumura, Li, Choi, Shen: "Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 11, pp. L1016-25, (2014) (PubMed).

Sanchez, Penfornis, Oskowitz, Boonjindasup, Cai, Dhule, Rowan, Kelekar, Krause, Pochampally: "Activation of autophagy in mesenchymal stem cells provides tumor stromal support." in: Carcinogenesis, Vol. 32, Issue 7, pp. 964-72, (2011) (PubMed).

Yee, Wilkinson, James, Ryan, Vousden: "PUMA- and Bax-induced autophagy contributes to apoptosis." in: Cell death and differentiation, Vol. 16, Issue 8, pp. 1135-45, (2009) (PubMed).

Harada, Willison, Sakakibara, Miyamoto, Fujita, Taniguchi: "Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated." in: Cell, Vol. 63, Issue 2, pp. 303-12, (1990) (PubMed).
抗原

ATG12 (Autophagy Related 12 (ATG12))

别名

ATG12 (ATG12 产品)

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

分子量

15113

基因ID

9140

NCBI登录号

NP_001264712, NP_004698

UniProt

O94817

途径

Autophagy