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ATG7 抗体 (C-Term)

Name: ATG7 抗体 (C-Term)
SKU: ABIN388523
Availability: OutOfStock

ATG7 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7474 unconjugated

Western blot analysis of G7L Pab 1813d in 293 cell line lysate (35 μg/lane). (ATG7 抗体 (C-Term))

Immunohistochemical analysis of paraffin-embedded human liver tissue using D performed on the Leica® BOND RXm. (ATG7 抗体 (C-Term))

IHC (p)

All lanes : Anti-ATG7 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. (ATG7 抗体 (C-Term))

All lanes : Anti-ATG7 Antibody (C-term) at 1:500 dilution Lane 1: Caki-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 40 μg per lane. (ATG7 抗体 (C-Term))

Immunofluorescent analysis of cells, using ATG7 Antibody (C-term) (ABIN388523 and ABIN2849643). (ATG7 抗体 (C-Term))

5 images

(2 references)

产品编号 ABIN388523

发货至: 中国

Datasheet as PDF

抗原 See all ATG7 抗体

ATG7 (ATG7 Autophagy Related 7 (ATG7))
抗原表位
16

16

15

8

6

6

6

6

5

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 540-569, C-Term
适用
116

38

26

2

1

1

1

1

1

1

1
人, 小鼠
宿主
104

13

2
兔
Compatible Secondaries
克隆类型
100

19
多克隆
标记
58

9

8

7

6

5

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG7 antibody is un-conjugated
应用范围
98

58

47

22

16

14

14

13

12

7

5

4

3

2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

C, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.

克隆位点

RB7474

亚型

Ig Fraction

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应用备注

IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:500. IHC-P-Leica: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Wu, Sun, Ma, Zhu, Zhang, Pan, Li, Liu, Diao, Li: "A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum." in: Scientific reports, Vol. 6, pp. 27332, (2018) (PubMed).

Ding, Shi, Zhou, Qiu, Xu, Dai, Shi, Wang, Ke, Wu, Fan: "Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9167-75, (2008) (PubMed).
抗原

ATG7 (ATG7 Autophagy Related 7 (ATG7))

别名

ATG7 (ATG7 产品)

别名

APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

分子量

77960

基因ID

10533

NCBI登录号

NP_001129503, NP_001138384, NP_006386

UniProt

O95352

途径

Response to Water Deprivation, Autophagy