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ATG5 抗体 (C-Term)

Name: ATG5 抗体 (C-Term)
SKU: ABIN388519
Availability: OutOfStock

ATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated

Western blot analysis of G5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates 1812b. (ATG5 抗体 (C-Term))

Fluorescent image of cells stained with ATG5 Antibody (C-term) (ABIN388519 and ABIN2849632). (ATG5 抗体 (C-Term))

Cos7, HE, MEF, and Hela cells left to right, respectively. (ATG5 抗体 (C-Term))

Western blot analysis of G5L (arrow) using rabbit polyclonal G5L Antibody 1812b. (ATG5 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ATG5 抗体 (C-Term))

IHC (p)

5 images

(29 references)

产品编号 ABIN388519

发货至: 中国

Datasheet as PDF

抗原 See all ATG5 抗体

ATG5 (ATG5 Autophagy Related 5 (ATG5))
抗原表位
16

12

7

6

6

5

5

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1
AA 209-238, C-Term
适用
108

47

40

6

6

5

5

4

4

4

4

3

3

2
人, 小鼠
宿主
83

22

4

1
兔
Compatible Secondaries
克隆类型
85

25
多克隆
标记
52

8

7

6

6

6

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG5 antibody is un-conjugated
应用范围
72

54

33

17

14

14

13

13

10

7

4

3

3

3

2

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.

克隆位点

RB7547

亚型

IgG

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anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibody
ATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibody
ATG5 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3C7A8 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibody
ATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibody
ATG5 适用: 人 WB, ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibody
ATG5 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30), (N-Term) antibody
ATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 28-275) antibody
ATG5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) antibody
KO Validated ATG5 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Zhang, Michel, Lenz, Friedel, Köster, dHedouville, Tönges, Urlaub, Bähr, Lingor, Koch: "Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration." in: Scientific reports, Vol. 6, pp. 37050, (2018) (PubMed).

Radogna, Cerella, Gaigneaux, Christov, Dicato, Diederich: "Cell type-dependent ROS and mitophagy response leads to apoptosis or necroptosis in neuroblastoma." in: Oncogene, Vol. 35, Issue 29, pp. 3839-53, (2017) (PubMed).

Kessel, Evans: "Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy." in: Photochemistry and photobiology, Vol. 92, Issue 4, pp. 620-3, (2017) (PubMed).

Zhang, Gao, Zhang, Song, Cheng, Zhou: "The germline-enriched Ppp1r36 promotes autophagy." in: Scientific reports, Vol. 6, pp. 24609, (2017) (PubMed).

Mathai, Meijer, Simonsen: "Studying Autophagy in Zebrafish." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Du, Ren, Yao, Duan, Zhao, Du, Xiao, Duan, Shi: "Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy." in: The international journal of biochemistry & cell biology, Vol. 90, pp. 17-28, (2017) (PubMed).

Paquet, Nicoll, Love, Mouton-Liger, Holmes, Hugon, Boche: "Downregulated apoptosis and autophagy after anti-Aβ immunotherapy in Alzheimer's disease." in: Brain pathology (Zurich, Switzerland), (2017) (PubMed).

Wang, Li, Wang, Ma, Chuai, Cao, Yang: "High glucose environment inhibits cranial neural crest survival by activating excessive autophagy in the chick embryo." in: Scientific reports, Vol. 5, pp. 18321, (2016) (PubMed).

Varga, Fodor, Vellai: "Autophagy in zebrafish." in: Methods (San Diego, Calif.), Vol. 75, pp. 172-80, (2015) (PubMed).

Ribas, Schnepf, Challagundla, Koch, Bähr, Lingor: "Early and sustained activation of autophagy in degenerating axons after spinal cord injury." in: Brain pathology (Zurich, Switzerland), Vol. 25, Issue 2, pp. 157-70, (2015) (PubMed).

Hsin, Ou, Wu, Jan, Hsiao, Lin, Ko: "GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells." in: Molecular pharmaceutics, Vol. 12, Issue 5, pp. 1534-43, (2015) (PubMed).

Xin, Xiao, Ma, He, Yao, Liu et al.: "Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related ..." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 26, pp. 95-102, (2014) (PubMed).

Degtyarev, Reichelt, Lin: "Novel quantitative autophagy analysis by organelle flow cytometry after cell sonication." in: PLoS ONE, Vol. 9, Issue 1, pp. e87707, (2014) (PubMed).

Yao, Jia, Shelby, Ganios, Feathers, Thompson, Zacks: "Circadian and noncircadian modulation of autophagy in photoreceptors and retinal pigment epithelium." in: Investigative ophthalmology & visual science, Vol. 55, Issue 5, pp. 3237-46, (2014) (PubMed).

Al-Zeer, Al-Younes, Lauster, Abu Lubad, Meyer: "Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins." in: Autophagy, Vol. 9, Issue 1, pp. 50-62, (2013) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

Chiu, Chen, Ho, Wang: "Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e31579, (2012) (PubMed).

Hu, Zhang, Zhang: "Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis." in: Autophagy, Vol. 7, Issue 12, pp. 1514-27, (2012) (PubMed).

Norberg, Karlsson, Korenovska, Szydlowski, Silberberg, Uhlén, Orrenius, Zhivotovsky: "Critical role for hyperpolarization-activated cyclic nucleotide-gated channel 2 in the AIF-mediated apoptosis." in: The EMBO journal, Vol. 29, Issue 22, pp. 3869-78, (2010) (PubMed).

Dou, Chattopadhyay, Pan, Guerriero, Jiang, Ballou, Yue, Lin, Zong: "The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy." in: The Journal of cell biology, Vol. 191, Issue 4, pp. 827-43, (2010) (PubMed).

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抗原

ATG5 (ATG5 Autophagy Related 5 (ATG5))

别名

ATG5 (ATG5 产品)

别名

APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

分子量

32447

基因ID

9474

NCBI登录号

NP_004840

UniProt

Q9H1Y0

途径

Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy