(3 references)-
- 抗原 See all GST-Tag products
- GST-Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GST-Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 免疫原
- This GST Tag antibody is generated from rabbits immunized with a recombinant full length of GST.
- 克隆位点
- RB2165
- 亚型
- Ig Fraction
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anti-GST-Tag antibody
适用: Schistosoma japonicum WB, ELISA 宿主: 小鼠 Monoclonal 9AT106 unconjugated
anti-GST-Tag antibody (Biotin)适用: Schistosoma japonicum WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-GST-Tag antibodyWB, IP 宿主: 小鼠 Monoclonal S-tag-05 unconjugated
anti-GST-Tag antibodyWB 宿主: 小鼠 Monoclonal 9AT106 unconjugated
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- 应用备注
- WB: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- 有效期
- 6 months
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: "Mammalian sterile 20-like kinase 1/2 inhibits the Wnt/?-catenin signalling pathway by directly binding casein kinase 1?." in: The Biochemical journal, Vol. 458, Issue 1, pp. 159-69, (2014) (PubMed).
: "Scd6 targets eIF4G to repress translation: RGG motif proteins as a class of eIF4G-binding proteins." in: Molecular cell, Vol. 45, Issue 2, pp. 244-54, (2012) (PubMed).
: "Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms." in: Molecular cell, Vol. 39, Issue 5, pp. 773-83, (2010) (PubMed).
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: "Mammalian sterile 20-like kinase 1/2 inhibits the Wnt/?-catenin signalling pathway by directly binding casein kinase 1?." in: The Biochemical journal, Vol. 458, Issue 1, pp. 159-69, (2014) (PubMed).
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- 抗原
- GST-Tag
- Abstract
- GST-Tag 产品
- 物质类
- Tag
- 背景
- Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
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