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HDAC9 抗体 (N-Term)

Name: HDAC9 抗体 (N-Term)
SKU: ABIN387960
Availability: OutOfStock

HDAC9 适用: 人 WB, IF, IP, IHC (p) 宿主: 兔 Polyclonal RB2582 unconjugated

Both anti-HDAC9 N-term ((ABIN387960 and ABIN2844679)) and C-term ((ABIN387961 and ABIN2844680)) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. (HDAC9 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (HDAC9 抗体 (N-Term))

IHC (p)

Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. (HDAC9 抗体 (N-Term))

This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (HDAC9 抗体 (N-Term))

Figure 1: Immunoblots for MITR ((ABIN387960 and ABIN2844679) HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. (HDAC9 抗体 (N-Term))

5 images

(3 references)

产品编号 ABIN387960

发货至: 中国

Datasheet as PDF

抗原 See all HDAC9 抗体

HDAC9 (Histone Deacetylase 9 (HDAC9))
抗原表位
13

9

7

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 2-32, N-Term
适用
78

28

20

6

4

4

3

3

2

2

2

1

1

1

1
人
宿主
70

8
兔
Compatible Secondaries
克隆类型
64

14
多克隆
标记
43

6

4

3

3

3

3

3

3

3

2

2
This HDAC9 antibody is un-conjugated
应用范围
51

37

33

20

20

9

7

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

C, M

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HDAC9.

克隆位点

RB2582

亚型

Ig Fraction

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anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody
HDAC9 适用: 人, 小鼠, 豚鼠, 绵羊 WB, IHC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) (AA 2-32) antibody
HDAC9 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) antibody
HDAC9 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 1F2 unconjugated Recombinant Antibody

anti-Histone Deacetylase 9 (HDAC9) (AA 343-569) antibody
HDAC9 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2B7C4 unconjugated

anti-Histone Deacetylase 9 (HDAC9) (AA 23-343) antibody
HDAC9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody
HDAC9 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) (pSer220), (pSer259) antibody
HDAC9 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody
HDAC9 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody
HDAC9 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:1,000. IP: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Salian-Mehta, Xu, McKinsey, Tobet, Wierman: "Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14045-56, (2015) (PubMed).

Sugo, Oshiro, Takemura, Kobayashi, Kohno, Uesaka, Song, Yamamoto: "Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons." in: The European journal of neuroscience, Vol. 31, Issue 9, pp. 1521-32, (2010) (PubMed).

Deng, Ewton, Mercer, Friedman: "Mirk/dyrk1B decreases the nuclear accumulation of class II histone deacetylases during skeletal muscle differentiation." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4894-905, (2005) (PubMed).
抗原

HDAC9 (Histone Deacetylase 9 (HDAC9))

别名

HDAC9 (HDAC9 产品)

别名

HD7 antibody, HD7b antibody, HD9 antibody, HDAC antibody, HDAC7 antibody, HDAC7B antibody, HDAC9B antibody, HDAC9FL antibody, HDRP antibody, MITR antibody, AV022454 antibody, D030072B18Rik antibody, HD7B antibody, Hdac7b antibody, Mitr antibody, mKIAA0744 antibody, hdac9 antibody, zgc:73392 antibody, TWIST antibody, HDAC9 antibody, DKFZp459E171 antibody, HDA09 antibody, HISTONE DEACETYLASE antibody, histone deacetylase 9 antibody, RGD1310748 antibody, RGD1563092 antibody, hdac9-A antibody, histone deacetylase 9 antibody, histone deacetylase 9b antibody, histone deacetylase 9 S homeolog antibody, HDAC9 antibody, Hdac9 antibody, hdac9b antibody, HDA9 antibody, hdac9.S antibody

背景

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.

分子量

111297

基因ID

9734

NCBI登录号

NP_001191073, NP_001191074, NP_001191075, NP_001191076, NP_001191077, NP_055522, NP_478056, NP_848510, NP_848512

UniProt

Q9UKV0

途径

Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development