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DNMT3B 抗体 (AA 389-417)

Name: DNMT3B 抗体 (AA 389-417)
SKU: ABIN387884
Availability: OutOfStock

DNMT3B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01906 unconjugated

All lanes : Anti-Dnmt3b Antibody at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 μg per lane. (DNMT3B 抗体 (AA 389-417))

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (ABIN387884 and ABIN2844100) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DNMT3B 抗体 (AA 389-417))

IHC (p)

Anti-Dnmt3b Antibody at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 μg per lane. (DNMT3B 抗体 (AA 389-417))

3 images

(12 references)

产品编号 ABIN387884

发货至: 中国

Datasheet as PDF

抗原 See all DNMT3B 抗体

DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))
抗原表位
18

16

7

7

5

3

2

2

1

1

1

1

1

1

1

1

1

1
AA 389-417
适用
85

53

32

3

3

2

2

2

2

2

2

1

1
人
宿主
81

6

2

1
兔
Compatible Secondaries
克隆类型
75

15
多克隆
标记
35

5

5

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This DNMT3B antibody is un-conjugated
应用范围
71

36

24

21

13

13

10

8

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b.

克隆位点

RB01906

亚型

Ig Fraction

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anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 1-150) antibody
DNMT3B 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 4D1A2E5 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 1-80) antibody
DNMT3B 适用: 人, 小鸡 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (N-Term) antibody
DNMT3B 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 1-100) antibody
DNMT3B 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (N-Term) antibody
DNMT3B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody
DNMT3B 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 1-80) antibody
DNMT3B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody
DNMT3B 适用: 人 WB, FACS, ICC, DB 宿主: 小鼠 Monoclonal 2280C3a unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (N-Term) antibody
DNMT3B 适用: 人, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Li, Ebert, Li et al.: "T cell receptor (TCR) and transforming growth factor ? (TGF-?) signaling converge on DNA (cytosine-5)-methyltransferase to control forkhead box protein 3 (foxp3) locus methylation and inducible ..." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 19127-39, (2013) (PubMed).

Chen, Wang, Hu, Chang, Liao, Dai, Juo: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, (2011) (PubMed).

Sohn, Park, Lee, Yang, Jang, Park, Kim, Lee, Sohn, Kim, Yoo, Choi, Bae, Yeom: "Functional switching of TGF-beta1 signaling in liver cancer via epigenetic modulation of a single CpG site in TTP promoter." in: Gastroenterology, Vol. 138, Issue 5, pp. 1898-908, (2010) (PubMed).

Lu, Markowetz, Unwin, Leek, Airoldi, MacArthur, Lachmann, Rozov, Maayan, Boyer, Troyanskaya, Whetton, Lemischka: "Systems-level dynamic analyses of fate change in murine embryonic stem cells." in: Nature, Vol. 462, Issue 7271, pp. 358-62, (2009) (PubMed).

Yamagata, Asada, Tamura, Lee, Maekawa, Taniguchi, Taketani, Matsuoka, Tamura, Sugino: "DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen." in: Human reproduction (Oxford, England), Vol. 24, Issue 5, pp. 1126-32, (2009) (PubMed).

Park, Sohn, Yu, Suh, Chung, Lee, Surzycki, Lee: "Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein." in: Gastroenterology, Vol. 132, Issue 4, pp. 1476-94, (2007) (PubMed).

Fabbri, Garzon, Cimmino, Liu, Zanesi, Callegari, Liu, Alder, Costinean, Fernandez-Cymering, Volinia, Guler, Morrison, Chan, Marcucci, Calin, Huebner, Croce: "MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 40, pp. 15805-10, (2007) (PubMed).

Koturbash, Boyko, Rodriguez-Juarez, McDonald, Tryndyak, Kovalchuk, Pogribny, Kovalchuk: "Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1831-8, (2007) (PubMed).

Tryndyak, Muskhelishvili, Kovalchuk, Rodriguez-Juarez, Montgomery, Churchwell, Ross, Beland, Pogribny: "Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis." in: Carcinogenesis, Vol. 27, Issue 8, pp. 1713-20, (2006) (PubMed).

Koturbash, Rugo, Hendricks, Loree, Thibault, Kutanzi, Pogribny, Yanch, Engelward, Kovalchuk: "Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo." in: Oncogene, Vol. 25, Issue 31, pp. 4267-75, (2006) (PubMed).

Brueckner, Garcia Boy, Siedlecki, Musch, Kliem, Zielenkiewicz, Suhai, Wiessler, Lyko: "Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases." in: Cancer research, Vol. 65, Issue 14, pp. 6305-11, (2005) (PubMed).

Pogribny, Koturbash, Tryndyak, Hudson, Stevenson, Sedelnikova, Bonner, Kovalchuk: "Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus." in: Molecular cancer research : MCR, Vol. 3, Issue 10, pp. 553-61, (2005) (PubMed).
抗原

DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

别名

Dnmt3b (DNMT3B 产品)

别名

DNMT3B antibody, LOC100218113 antibody, ICF antibody, ICF1 antibody, M.HsaIIIB antibody, MmuIIIB antibody, cb91 antibody, dnmt3bl antibody, dnmt7 antibody, sb:cb91 antibody, wu:fb16h07 antibody, DNA methyltransferase 3 beta antibody, DNA methyltransferase 3B antibody, DNA (cytosine-5-)-methyltransferase 3 beta, duplicate a antibody, DNMT3B antibody, Dnmt3b antibody, dnmt3b antibody, dnmt3ba antibody

背景

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.

分子量

95751

基因ID

1789

NCBI登录号

NP_001193984, NP_001193985, NP_008823, NP_787044, NP_787045, NP_787046

UniProt

Q9UBC3