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HA-Tag 抗体

Name: HA-Tag 抗体
SKU: ABIN387848
Availability: OutOfStock

WB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated

Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. (HA-Tag 抗体)

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. (HA-Tag 抗体)

ChIP

Western blot analysis of Tag-HA. (HA-Tag 抗体)

4 images

(22 references)

产品编号 ABIN387848

发货至: 中国

Datasheet as PDF

抗原 See all HA-Tag products

HA-Tag

适用

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宿主
76

73

13

8

1
兔
Compatible Secondaries
克隆类型
87

81
多克隆
标记
66

15

13

11

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HA-Tag antibody is un-conjugated
应用范围
129

57

37

24

18

16

14

13

9

6

6

6

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

序列

YPYDVPDYA

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

克隆位点

RB0411

亚型

Ig Fraction
anti-HA-Tag antibody
WB, ELISA, IP, IF, IHC 宿主: 小鼠 Monoclonal HA-C5 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody
WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP

anti-HA-Tag antibody
适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated

anti-HA-Tag antibody
ELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated

anti-HA-Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 12CA5 unconjugated

anti-HA-Tag antibody
WB, IP, IF, EIA 宿主: 小鼠 Monoclonal 26D11 unconjugated

应用备注

IF: 1:10~50. CHIP: 1:100. WB: 1:1000. WB: 1:1000. WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Wu, Sun, Ma, Zhu, Zhang, Pan, Li, Liu, Diao, Li: "A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum." in: Scientific reports, Vol. 6, pp. 27332, (2018) (PubMed).

Baruah, Hitzman, Zhang, Chaudhuri, Mastej, Wary: "The allosteric glycogen synthase kinase-3 inhibitor NP12 limits myocardial remodeling and promotes angiogenesis in an acute myocardial infarction model." in: The Journal of biological chemistry, Vol. 292, Issue 50, pp. 20785-20798, (2018) (PubMed).

Simkin, Robin, Giuliano, Vukolic, Moceri, Guy, Wagner, Lacampagne, Allard, Bendahhou: "Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo." in: Cell and tissue research, Vol. 371, Issue 2, pp. 309-323, (2017) (PubMed).

Di, Wang, Han, Fu, Duerfeldt, Blagg, Mu: "Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9526-39, (2016) (PubMed).

Koo, Yue, Deng, Khuri, Sun: "mTOR Complex 2 Stabilizes Mcl-1 Protein by Suppressing Its Glycogen Synthase Kinase 3-Dependent and SCF-FBXW7-Mediated Degradation." in: Molecular and cellular biology, Vol. 35, Issue 13, pp. 2344-55, (2015) (PubMed).

Han, Guan, Wang, Hatzoglou, Mu: "L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated ?1(D219N) Subunits of GABAA Receptors." in: ACS chemical biology, (2015) (PubMed).

Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, (2015) (PubMed).

Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, (2015) (PubMed).

Oh, Yue, Wang, Tong, Chen, Khuri, Sun: "Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling." in: Oncotarget, (2015) (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, (2015) (PubMed).

Zhao, Yue, Khuri, Sun: "mTOR complex 2 is involved in regulation of Cbl-dependent c-FLIP degradation and sensitivity of TRAIL-induced apoptosis." in: Cancer research, Vol. 73, Issue 6, pp. 1946-57, (2013) (PubMed).

Oh, Yue, Zhou, Balko, Black, Owonikoko, Khuri, Sun: "Oncogenic Ras and B-Raf proteins positively regulate death receptor 5 expression through co-activation of ERK and JNK signaling." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 257-67, (2012) (PubMed).

Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, (2012) (PubMed).

Zhao, Yue, Lonial, Khuri, Sun: "The NEDD8-activating enzyme inhibitor, MLN4924, cooperates with TRAIL to augment apoptosis through facilitating c-FLIP degradation in head and neck cancer cells." in: Molecular cancer therapeutics, Vol. 10, Issue 12, pp. 2415-25, (2011) (PubMed).

Fan, Li, Yue, Khuri, Sun: "The eIF4E/eIF4G interaction inhibitor 4EGI-1 augments TRAIL-mediated apoptosis through c-FLIP Down-regulation and DR5 induction independent of inhibition of cap-dependent protein translation." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 4, pp. 346-56, (2010) (PubMed).

Guo, Wang, Wang, Huang, Liang, Chen: "Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis." in: Plant & cell physiology, Vol. 50, Issue 9, pp. 1681-94, (2009) (PubMed).

Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, (2009) (PubMed).

Lin, Liu, Yue, Benbrook, Berlin, Khuri, Sun: "Involvement of c-FLIP and survivin down-regulation in flexible heteroarotinoid-induced apoptosis and enhancement of TRAIL-initiated apoptosis in lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 11, pp. 3556-65, (2008) (PubMed).

Wang, Kwak, Zeng, Ellis, Chen, Schiefelbein, Chen: "TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis." in: Development (Cambridge, England), Vol. 134, Issue 21, pp. 3873-82, (2007) (PubMed).

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抗原

HA-Tag

Abstract

HA-Tag 产品

物质类

Tag

背景

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.