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His Tag 抗体

Name: His Tag 抗体
SKU: ABIN387750
Availability: OutOfStock

适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

Anti-HIS Tag at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 μg per lane. (His Tag 抗体)

Anti-HIS Tag at 1:8000 dilution + 12-tag lysate Lysates/proteins at 20 ng per lane. (His Tag 抗体)

Western blot analysis of lysates from 12tag protein , This demonstrates the His tagged antibody detected the His tagged protein (arrow). (His Tag 抗体)

3 images

(38 references)

产品编号 ABIN387750

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag
适用
20

5

4

1

1

1
人
宿主
90

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
92

76

10
单克隆
标记
78

21

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
140

91

39

27

24

18

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB)

序列

HHHHHH

纯化方法

This antibody is purified through a protein G column, followed by dialysis against PBS.

免疫原

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

克隆位点

6AT18

亚型

IgG1

Top Product

Discover our top product His Tag Primary Antibody
anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FLISA, FM 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

anti-His Tag antibody
WB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated

应用备注

WB: 1:8000. WB: 1:1,000. WB: 1:2000

限制

仅限研究用
状态

Liquid

缓冲液

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Khan, Faure, Chapman, Blancaflor: "A chemical genetic screen uncovers a small molecule enhancer of the N-acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis." in: Scientific reports, Vol. 7, pp. 41121, (2018) (PubMed).

Sun, Ji, Liang, Du, Jiang, Wang, Kai, Zhang, Zhai, Chen, Wang, Leng: "Suppressing ABA uridine diphosphate glucosyltransferase (SlUGT75C1) alters fruit ripening and the stress response in tomato." in: The Plant journal : for cell and molecular biology, Vol. 91, Issue 4, pp. 574-589, (2018) (PubMed).

Wang, Gu, Zhuang, Zhong, Yang, Wang: "Screening and Molecular Evolution of a Single Chain Variable Fragment Antibody (scFv) against Citreoviridin Toxin." in: Journal of agricultural and food chemistry, Vol. 64, Issue 40, pp. 7640-7648, (2017) (PubMed).

Wang, Zhao: "A simple strategy for extracellular production of CotA laccase in Escherichia coli and decolorization of simulated textile effluent by recombinant laccase." in: Applied microbiology and biotechnology, Vol. 101, Issue 2, pp. 685-696, (2017) (PubMed).

Olp, Zhu, Smith: "Metabolically Derived Lysine Acylations and Neighboring Modifications Tune the Binding of the BET Bromodomains to Histone H4." in: Biochemistry, Vol. 56, Issue 41, pp. 5485-5495, (2017) (PubMed).

Rath, Hilton, Sisco, Van Horn: "Implications of Human Transient Receptor Potential Melastatin 8 (TRPM8) Channel Gating from Menthol Binding Studies of the Sensing Domain." in: Biochemistry, Vol. 55, Issue 1, pp. 114-24, (2016) (PubMed).

Tian, Qu, Bao, Gao, Liu, Wang, Sun, Ding, Yu: "Construction of pTM series plasmids for gene expression in Brucella species." in: Journal of microbiological methods, Vol. 123, pp. 18-23, (2016) (PubMed).

Militi, Maywood, Sandate, Chesham, Barnard, Parsons, Vibert, Joynson, Partch, Hastings, Nolan: "Early doors (Edo) mutant mouse reveals the importance of period 2 (PER2) PAS domain structure for circadian pacemaking." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 10, pp. 2756-61, (2016) (PubMed).

Brown, Nicholson, Arama, Mercer, Thomson, Jovanovic: "γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains." in: The Journal of biological chemistry, Vol. 291, Issue 27, pp. 13926-42, (2016) (PubMed).

Kim, Pai, Sato, Person, Nakamura, Macdonald: "Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression." in: PLoS genetics, Vol. 11, Issue 2, pp. e1004992, (2015) (PubMed).

Grigoroudis, McInnes, Premnath, Kontopidis: "Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones." in: Protein expression and purification, Vol. 113, pp. 8-16, (2015) (PubMed).

Yarosh, Tapescu, Thompson, Qiu, Mallory, Fu, Lynch: "TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells." in: Nucleic acids research, Vol. 43, Issue 18, pp. 9006-16, (2015) (PubMed).

Lv, Lei, Hong, Wang, Zhang, Zhou, Sun, Ma, Shen, Zhu: "Venom allergen 5 is Associated With Deltamethrin Resistance in Culex pipiens pallens (Diptera: Culicidae)." in: Journal of medical entomology, Vol. 52, Issue 4, pp. 672-82, (2015) (PubMed).

Bhandari, Lopez-Sanchez, To, Lo, Aznar, Leyme, Gupta, Niesman, Maddox, Garcia-Marcos, Farquhar, Ghosh: "Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 35, pp. E4874-83, (2015) (PubMed).

Shi, Zhang, Guo, Zhang, Hu, Xi, Miao, Li: "131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma." in: Oncology reports, (2015) (PubMed).

Liu, Novick: "Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p." in: The Journal of cell biology, Vol. 207, Issue 1, pp. 59-72, (2014) (PubMed).

Hufnagel, DePas, Chapman: "The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli." in: Journal of bacteriology, Vol. 196, Issue 21, pp. 3690-9, (2014) (PubMed).

Faure, Nagarajan, Hwang, Montgomery, Khan, John, Koulen, Blancaflor, Chapman: "Synthesis of phenoxyacyl-ethanolamides and their effects on fatty acid amide hydrolase activity." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9340-51, (2014) (PubMed).

Malmirchegini, Sjodt, Shnitkind, Sawaya, Rosinski, Newton, Klebba, Clubb: "Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes." in: The Journal of biological chemistry, Vol. 289, Issue 50, pp. 34886-99, (2014) (PubMed).

Wang, Xiang, Zhang, Chen, Zhong, Wang: "Development of a functional antibody by using a green fluorescent protein frame as the template." in: Applied and environmental microbiology, Vol. 80, Issue 14, pp. 4126-37, (2014) (PubMed).

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抗原

His Tag

Abstract

His Tag 产品

物质类

Tag

背景

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

基因ID

12406