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HA-Tag 抗体 (HRP)

WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP
产品编号 ABIN387712
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This HA-Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    品牌
    THE™
    序列
    YPYDVPDYA
    特异性
    THETM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
    产品特性
    The antibody is suitable for detecting the expression level of HA in fusion proteins. Do not add azide as it inhibits HRP activity.
    纯化方法
    Protein A affinity column
    免疫原
    A synthetic peptide (YPYDVPDYA) coupled - KLH
    克隆位点
    5E11D8
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-0.5 µg/mL
    Western blot: 0.1-0.5 µg/mL
    Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    0.5 mg/mL
    缓冲液
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Haac, Anderson, Eggleston, Myles, Adelman: "The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3688-700, (2015) (PubMed).

  • 抗原
    HA-Tag
    别名
    HA Tag (HA-Tag 产品)
    物质类
    Tag
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