Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.
产品特性
Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.
纯化方法
Protein A affinity column
免疫原
A synthetic peptide corresponding - AA 410-419 (EQKLISEEDL) coupled - KLH
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product. ELISA: 0.05- 0.2 µg/mL Western blot: 0.1-1 µg/mL Immunoprecipitation (IP): 1 µg/mL Immunofluorescent staining: 1 µg/mL Other applications: user-optimized
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing cycles.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.