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His Tag 抗体

Name: His Tag 抗体
SKU: ABIN387699
Availability: OutOfStock

WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

Lane 1-3: Multiple Tag Cell Lysate (ABIN1536505) Lane 4: Negative controlPrimary Antibody: 1 µg/mL Anti-His Monoclonal Antibody (ABIN387699) Secondary Antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (ABIN398387, 1: 10,000) (His Tag 抗体)

Lane 1: 6x His Protein Ladder. (His Tag 抗体)

3 images

(30 references)

产品编号 ABIN387699

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
90

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
92

76

10
单克隆
标记
78

21

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
140

90

38

27

23

17

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

序列

HHHHHH

特异性

THE^TMHis Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

产品特性

Anti-His mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. Anti-His mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. Anti-His mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.

纯化方法

Protein A affinity column

免疫原

A synthetic peptide HHHHHH coupled - KLH

克隆位点

6G2A9

亚型

IgG

Top Product

Discover our top product His Tag Primary Antibody
anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FLISA, FM 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

anti-His Tag antibody
WB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-0.2 µg/mL
Immunoprecipitation (IP): 1 µg/mL
Immunofluorescent staining: 1 µg/mL
Flow cytometry: 1 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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抗原

His Tag

Abstract

His Tag 产品

物质类

Tag

背景

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at successfully isolated an antibody against His-tag. Anti-His mAb (subtype IgG1) has very high affinity. Tests performed at show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.