Applications: ELISA - Quality tested FLISA - Quality tested FC - Quality tested WB - Reported in literature , PLA - Reported in literature
Working Dilutions: Flow Cytometry FITC conjugate 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA Purified (UNLB) antibody 1 g/mL HRP conjugate 1:4,000 - 1:8,000 AP conjugate 1:1,000 - 1:2,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate 1 g/mL
样本量
1 mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
储存液
Without preservative
注意事项
Each reagent is stable for the period shown on the bottle label if stored as directed.
Anti-Biotin can be used for indirect immuno-staining when working with a biotinylated primary antibody. It can be used for simultaneous staining of several biotinylated antibodies as one of the parameters in certain assay platforms (such as Luminex). It is an alternative technology to magnify the signal and to reduce background caused by avidin or its derivatives.