Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugate 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
样本量
1 mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
储存液
Without preservative
注意事项
Each reagent is stable for the period shown on the bottle label if stored as directed.
CD94 is expressed on the surface of NK cells, NK T cells and some memory phenotype CD8 + cells. When CD94 is paired with NKG2A, C, or E subunit, it binds the nonclassical MHC class Ib molecule Qa-1 b and results in a CD94 high population by flow cytometry. Approximately half of NK cells do not express NKG2A, C or E, these cells express CD94 on the surface in a form that does not bind Qa-1 b and is believed to be nonfunctional, producing a CD94 low population by flow cytometry. CD94 is thought to play a role in the adhesion and activation of the NK cell lineage.