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- 抗原 See all Sca-1/Ly-6A/E (Ly6a) 抗体
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Sca-1/Ly-6A/E antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa
- 产品特性
- Rat Anti-Mouse Ly-6A/E-APC
- 纯化方法
- Purified
- 免疫原
- IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
- 克隆位点
- D7
- 亚型
- IgG2a
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- 应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- 说明
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In vitro lymphocyte activation
- 样本量
- 1 mL
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C
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The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).
: "Irradiated stem cells and ageing of the haematopoietic system." in: Radiation and environmental biophysics, (2012) (PubMed).
: "
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The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).
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- 抗原
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
- 别名
- Ly-6A/E (Ly6a 产品)
- 别名
- Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody
- 背景
- Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E + resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E + lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.
- 途径
- Sensory Perception of Sound, Activated T Cell Proliferation
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