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Sca-1/Ly-6A/E 抗体 (APC)

Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 APC
产品编号 ABIN375409
发货至: 中国
  • 抗原 See all Sca-1/Ly-6A/E (Ly6a) 抗体
    Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
    适用
    • 52
    • 6
    • 1
    小鼠
    宿主
    • 32
    • 25
    大鼠
    克隆类型
    • 38
    • 17
    单克隆
    标记
    • 14
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sca-1/Ly-6A/E antibody is conjugated to APC
    应用范围
    • 32
    • 15
    • 12
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    特异性
    Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa
    产品特性
    Rat Anti-Mouse Ly-6A/E-APC
    纯化方法
    Purified
    免疫原
    IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
    克隆位点
    D7
    亚型
    IgG2a
  • 应用备注
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    说明

    In vitro lymphocyte activation

    样本量
    1 mL
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C
  • Filip, Mokrý, Vávrová, Sinkorová, Mičuda, Sponer, Filipová, Hrebíková, Dayanithi: "The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).

    Vávrová, Sinkorová, Rezáčová, Tichý, Filip, Mokrý, Lukášová: "Irradiated stem cells and ageing of the haematopoietic system." in: Radiation and environmental biophysics, (2012) (PubMed).

  • 抗原
    Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
    别名
    Ly-6A/E (Ly6a 产品)
    别名
    Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody
    背景
    Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E + resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E + lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.
    途径
    Sensory Perception of Sound, Activated T Cell Proliferation
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