SMN1 / SMN2 (all Isoforms), (Internal Region) 抗体
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- 抗原
- SMN1 / SMN2
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抗原表位
- all Isoforms, Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 序列
- C-DESENSRSPG NKSDN
- 特异性
- This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Synthetic peptide corresponding to internal region of Human SMN1+SMN2 according to NP_075012.1, NP_000335.1, NP_075013.1, NP_075014.1, NP_075015.1, NP_059107.1.
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anti-SMN1 / SMN2 (AA 22-52), (N-Term) antibody
适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SMN1 / SMN2 antibody适用: 人 WB, IHC (p), ICC 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-SMN1 / SMN2 (Internal Region) antibodyVerified 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-SMN1 / SMN2 (AA 51-100) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SMN1 / SMN2 (AA 22-52), (N-Term) antibody适用: 人 WB 宿主: 小鼠 Monoclonal 2B10 unconjugated
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- 应用备注
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Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 28.6 kDa according to NP_075012.1 and NP_075014.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- SMN1 / SMN2
- 背景
- The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly.Synonyms: Component of gems 1, Gemin-1, Gemin1, SMN, SMNC, SMNT, Survival motor neuron protein
- 基因ID
- 6606
- NCBI登录号
- NP_000335
- UniProt
- Q16637
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