Nanog 抗体 (Internal Region)
(8 references)-
- 抗原 See all Nanog (NANOG) 抗体
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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抗原表位
- Internal Region
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适用
- 人, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Nanog antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- C-QNQRMKSKRW QKNN
- 特异性
- This antibody is specific to NANOG.
- 交叉反应 （详细）
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Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Pig. - 纯化方法
- Ammonium sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide from the internal region of the protein sequence according to NP_079141.2.
- Top Product
- Discover our top product NANOG Primary Antibody
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anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 101-200) antibodyNANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-305) antibodyNANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 71-170) antibodyNANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 15-49) antibodyNANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Nanog Homeobox (NANOG) (Internal Region) antibodyVerified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-100) antibodyNANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 20-166) antibodyNANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated
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- 应用备注
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Peptide ELISA: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 38 kDa band observed in Human Ovary lysates(calculated MW of 34.8 kDa according to NP_079141.1). Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stemcells are stained for Nanog (green). (See also Ref.2)This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).
: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).
: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).
: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).
: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).
: "Lifestyle impacts on the aging-associated expression of biomarkers of DNA damage and telomere dysfunction in human blood." in: Aging cell, Vol. 9, Issue 4, pp. 607-15, (2010) (PubMed).
: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).
: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, (2008) (PubMed).
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: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).
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- 抗原
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- 别名
- NANOG (NANOG 产品)
- 背景
- Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG
- 基因ID
- 79923, 9606
- UniProt
- Q9H9S0
- 途径
- Stem Cell Maintenance
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