DAZL 抗体 (Internal Region)
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- 抗原 See all DAZL 抗体
- DAZL (Deleted in Azoospermia-Like (DAZL))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, Cow, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DAZL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- C-GNGPQKKSVD R
- 特异性
- This antibody recognizes DAZL.
- 交叉反应 （详细）
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Species reactivity (expected):Rat, Canine, Bovine.
Species reactivity (tested):Human, Mouse. - 纯化方法
- Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Synthetic peptide corresponding to the internal sequence of Human DAZL according to NP_001342.2 Genename: DAZL
- Top Product
- Discover our top product DAZL Primary Antibody
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anti-Deleted in Azoospermia-Like (DAZL) (AA 186-295) antibody
DAZL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (Internal Region) antibodyVerified DAZL 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (AA 130-250) antibodyDAZL 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (AA 1-295) antibodyDAZL 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (AA 218-267) antibodyDAZL 适用: 人, 小鼠, 大鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (C-Term) antibodyDAZL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Deleted in Azoospermia-Like (DAZL) (C-Term) antibodyDAZL 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: 1/64000. Western blot: Approx 35 kDa band observed in human ovary and human testes lysates(calculated MW of 33.5 kDa according to NP_001342.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Mouse Testis shows cytoplasm staining ofspermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatidsbut this additional staining is not in agreement with results obtained by another antibody(Ruggiuet al, Nature.1997 Sep 4,389(6646): 73-7. PMID: 9288969). Data kindly provided by J. S. Martins, MRC, Edinburgh, UK. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
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- 抗原
- DAZL (Deleted in Azoospermia-Like (DAZL))
- 别名
- DAZL (DAZL 产品)
- 背景
- DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.Synonyms: DAZ homolog, DAZ-like autosomal, DAZH, DAZL, DAZL1, DAZLA, Deleted in azoospermia-like, Deleted in azoospermia-like 1, SPGY-like-autosomal, SPGYLA
- 基因ID
- 1618
- NCBI登录号
- NP_001342
- UniProt
- Q92904
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