Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 12 kDa band observed in Human Brain lysates (calculated MW of 12.2 kDa accordingto NP_002845.1). Approx 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfectedwith human PVALB, but not in untransfected rat heart cells. (1) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.