CPEB1
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 647
应用备注
Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL. Approx 65-70 kDa band observed in Human Brain lysates(predicted MW of 60 kDa according to NP_085097). Please note this band was always accompanied by a slightly stonger band at approx 40 kDaand further both bands could be blocked by incubation with the immunising peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CPEB1
(Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))
The regulated translation of messenger RNA is essential for cell cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, which is one mechanism of controlling translation, is driven by CPEB1 (Cytoplasmic Polyadenylation Element Binding protein), a highly conserved, sequence specific RNA binding protein that binds to the cytoplasmic polyadenylation element (CPE) and modulates translational repression and mRNA localisation. CPEB1 can assemble bothe translational activation and repression complexes via its binding to the CPE. In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB1.Synonyms: CPE-BP1, CPE-binding protein 1, Cytoplasmic polyadenylation element-binding protein 1, h-CEBP, hCPEB-1