ACTR1A 抗体 (C-Term)
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- 抗原 See all ACTR1A 抗体
- ACTR1A (Alpha Centractin (ACTR1A))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ACTR1A antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- C-YEEDGARSIH RKT
- 特异性
- This antibody detects Centractin alpha at C-term.
- 交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, PigSpecies reactivity (tested):Human
- 纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- 免疫原
- Peptide from C Terminus of the protein sequence according to NP_005727.1 Genename: ACTR1A
- Top Product
- Discover our top product ACTR1A Primary Antibody
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anti-Alpha Centractin (ACTR1A) (AA 187-376) antibody
ACTR1A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alpha Centractin (ACTR1A) (C-Term) antibodyVerified ACTR1A 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Alpha Centractin (ACTR1A) (AA 279-375) antibodyACTR1A 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Alpha Centractin (ACTR1A) (AA 216-265) antibodyACTR1A 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, Pig, 豚鼠, 斑马鱼, Bat, Hamster, 猴, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Alpha Centractin (ACTR1A) antibodyACTR1A 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx.45 kDa band observed in Human Kidney lysates(calculated MW of 42.5 kDa according to NP_005727.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- 抗原
- ACTR1A (Alpha Centractin (ACTR1A))
- 别名
- Centractin alpha (ACTR1A 产品)
- 背景
- The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50 % amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.Synonyms: ACTR1A, ARP1, Actin-RPV, CTRN1, Centrosome-associated actin homolog
- 基因ID
- 10121
- NCBI登录号
- NP_005727
- UniProt
- P61163
- 途径
- M Phase
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