Peptide ELISA: 1/32000. Western blot: 0,3 - 1,0 μg/mL. Approx 30 kDa band observed in Hela and Human Lunglysates (predicted MW of 27.0 kDa according to NP_006401). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
TIP30 is a member of the short-chain dehydrogenases/reductases (SDR) family. It is a serine/threonine kinase that phosphorylates the C-terminal domain (CTD) of the largest RNA polymerase II subunit and induces the expression of apoptosis related genes Bad and Siva, as well as metastasis suppressor NM23 H2. It also interacts with an estrogen receptor alpha-interacting coactivator CIA and regulates ERalpha-mediated c-myc transcription. This protein is also known to bind the activation domain of HIV-1 Tat.Synonyms: 30 kDa HIV-1 TAT-interacting protein, CC3, HIV-1 TAT-interactive protein 2, Oxidoreductase HTATIP2, TIP30