This antibody detects GIPC1 at C-term. It is expected to recognize isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
交叉反应 (详细)
Species reactivity (tested):Human
纯化方法
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
免疫原
Peptide from C Terminus of the protein sequence according to NP_005707.1, NP_974197.1, NP_974199.1, NP_974196.1, NP_974198.1, NP_974223.1. Genename: GIPC1
Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.03-0.1 μg/mL. Approx 38 kDa and strong 23-28 kDa bands observed inlysates of cell line HeLa, approx 28 kDa also observed in human pancreas, kidney andskeletal muscle lysates (calculated MW of 36.8 kDa according to NP_005707.1,NP_974197.1 and NP_974199.1 and 26.8 kDa according to NP_974198.1 and NP_974223.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
抗原
GIPC1
(GIPC PDZ Domain Containing Family, Member 1 (GIPC1))